StellarTaq™ DNA Polymerase (DNAP) is engineered for extreme inhibitor tolerance, speed, and specificity. The polymerase catalyzes 5′ → 3′ DNA synthesis, has 5′ → 3′ exonuclease activity, and is deficient in 3′ → 5′ exonuclease activity making it suitable for probe digestion. It amplifies uracil-containing templates, incorporates modified bases, and performs A-tailing on DNA products. Available in hot start, non-hot start, and glycerol-free formats.

• Extreme inhibitor tolerance offers robust amplification across a range of clinical sample types, including urine, blood, sputum and bile
• High speed enables fast PCR applications
• Hot start mechanism ensures high specificity
• Custom formats, including high concentrate and glycerol-free, support lyophilization

ESCMID 2025 poster: The use of engineered reverse transcriptase and Taq DNA polymerase variants for improved activity and inhibitor tolerance in POC MDx applications(external link)

• Pathogen detection, including infectious diseases
• Fast PCR
• RT-qPCR

• PCR amplification of DNA fragments ≤ 5 kb
• Probe and intercalating dye-based qPCR
• PCR applications where biological inhibitors are present and specificity is important

Unit definition: One unit of StellarTaq DNA Polymerase incorporates 16 nmol of dNTPs into a DNA template in 30 minutes at 72°C

Reaction conditions* (materials not included in this kit):

• 20 mM Tris-HCl, pH 8.3
• 100 mM KCl
• 0.004% Tween 20

* Baseline reaction condition, optimization is required. See technical guide for more information.

Enzyme storage buffer:

• Standard: 50 mM Tris-HCl, pH 7.5, 100 mM KCl, 0.1 mM EDTA, 50% Glycerol, 0.05% Tween 20
• Glycerol-free: 50 mM Tris-HCl, pH 7.5, 300 mM KCl, 0.05% Tween 20