SpectraDyes for Fluorescent Western Blotting

Superior Sensitivity & Signal-to-Noise Ratio

• Brighter Fluorescence: SpectraDyes are engineered for high quantum yield, producing stronger signals at lower antibody concentrations compared to traditional dyes (e.g., AlexaFluor, Cy dyes).
• Low Background: Cross-adsorbed to minimize non-specific binding, reducing background noise in complex samples (e.g., cell lysates, tissue extracts).
• Wide Dynamic Range: Detects both high- and low-abundance proteins without signal saturation, ideal for quantitative Western blotting.

• Secondary antibodies labeled with fluorophores that emit light in visible and near-infrared wavelengths

  AzureSpectra 490, 550, 650, 700 and 800 secondary antibodies offer unparalleled sensitivity and performance for immunoblotting applications, especially when used in conjunction with Azure Imaging Systems(external link).
  
  Due to low background autofluorescence in the near-infrared region, AzureSpectra 700 and 800 secondary antibodies can produce higher signal-to-noise ratios. Choose the label and species best suited for your application. Each high-quality antibody offers unparalleled sensitivity and performance for immunoblotting applications.
  
  AzureSpectra Antibodies are IgG specific. They are polyclonal and bind to both the heavy and light chains of the primary antibody.