Enhanced protein transfer for improved sensitivity.
Achieve improved detection of low-abundance and posttranslationally modified proteins with Advansta's proprietary FLASHBlot Transfer Buffer. High-efficiency protein transfer and increased protein retention on the membrane add up to more sensitive Western blots.
- High Performance – increased protein retention on the membrane results in more sensitive detection of low abundance and post-translationally modified proteinss
- Efficient – enhanced transfer efficiency for proteins of all sizes
- Fast – transfer proteins in less than 20 minutes vs. several hours required with traditional transfer buffers
- Convenient – use your existing wet transfer electrophoresis apparatus
Complete transfer in less than 20 minutes. Transfer of CEA (high MW) and GAPDH (low MW) proteins was compared using FLASHBlot and Towbin transfer buffers. Following Western blot analysis, the data show equivalent protein transfer in 15 minutes with FLASHBlot buffer vs. 60 minutes with Towbin buffer.
More efficient transfers with FLASHBlot buffer. After 20-minute transfers, Western blot analysis shows that both transferrin and GAPDH are more efficiently transferred using FLASHBlot buffer than using Towbin buffer.
Shorter transfer times. FLASHBlot achieves complete protein transfer in less than 20 minutes using any traditional wet transfer apparatus.