Short Read Eliminators
Circulomics Short Read Eliminator Kit Family (SRE XS, SRE, SRE XL) can be used for rapid high-pass size selection of high molecular weight (HMW) DNA samples.
This method can significantly enhance mean read length by progressively depleting short DNA up to 10 kb (SRE XS), 25 kb (SRE), or 40 kb (SRE XL) in length.
Read length N50 can be increased by up to 10 – 30 kb depending on input sample quality.
The kits use a centrifugation procedure similar to standard ethanol precipitation techniques.
They have been thoroughly tested on Oxford Nanopore MinION/GridION/PromethION.
Protocols for PacBio Sequel applications will be released shortly.
If you would like to know more about the SRE kits, click here to download the handbook.
Figure 1. 1% Agarose gel separation of size-selected DNA with size cutoffs demonstrated using a spiked-in ladder (Thermo Scientific GeneRuler 1 kb Plus, #SM1334).
Input is 50 ng/µL gDNA extracted from GM12878 cells using Nanobind CBB Big DNA Kit + 20 ng/µL ladder.