Get a complete view of transcript isoform diversity with PacBio long-read sequencing.
Single Molecule, Real-Time (SMRT) Sequencing and Iso-Seq analysis allow you to generate full-length cDNA sequences — no assembly required — to characterize transcript isoforms within targeted genes or across an entire transcriptome so that you can easily and affordably:
- Discover new genes, transcripts and alternative splicing events
- Improve genome annotation to identify gene structure, regulatory elements and coding regions
- Increase the accuracy of RNA-seq quantification with isoform-level resolution
No need for short read-lenght polishing, the Iso-Seq method employs high-fidelity (>99% accurate) long reads to survey transcript diversity, either broadly or in a targeted fashion, revealing key information about the frequency and types of alternative transcription to improve genome annotation and gene discover.
Priced from USD2900 per sample, click here to get a quote