Combinatorial Variant Libraries

Twist Bioscience's unique capability to synthesize very large numbers of high quality oligos with our massively parallel silicon-based DNA synthesis platform, combined with Twist's well established molecular biology expertise, allow the controlled fabrication of highly diverse gene mutant libraries with highly specific, user--defined, composition.

Product Specs

• Product Format: Linear double stranded DNA, NGS-verified
• Delivery and Yield: All variants pooled in a single tube, up to 1 μg (depending on length of fragment)
• Price: Project dependent
• Turnaround Time: Project dependent

KEY BENEFITS

High Diversity Precision

• Multi-variant domains in single or multiple scaffolds (>10^10 variants)
• Precise control over codon usage (all 64 codons), amino acid distribution, and length variation

Verified Quality

• Rigorous quality control, including NGS-verification of modified regions
• Sequence variant ratios documented

Flexibility

• Design all sequences, single or multiple domains and combinations - single, pairwise, or triple variants
• Modular synthesis system enables iteration and of future libraries