PCR Biosystems’ Fluorescent Dye is a nucleic acid dye that becomes highly f luorescent upon binding to dsDNA. It is provided as a 20x stock reagent. The dye is ideal for a wide variety of applications including qPCR, high resolution DNA melt curve analysis and isothermal amplification. It is a green fluorescent dye with an excitation maximum at 482 nm and fluorescent emission maximum at 512 nm, so it can be visualized in every qPCR instrument by selecting the FAM channel. The dye is particularly suitable for use with the IsoFast™ Bst Polymerase products, catalogue number PB80.10-01 and PB80.10-08.